MoBiTec - Product Highlights - NEWS

EHEC Real Time PCR Kit, CE Marked

Intended Use
Enterohemorrhagic E. Coli (EHEC) real time PCR kit is used for the detection of Enterohemorrhagic E. Coli (EHEC) in excreta or water samples by using real time PCR systems.

Introduction
Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is the serotype most commonly associated with hemorrhagic colitis and the hemolytic uremic syndrome. It is phenotypically distinct from typical E. coli, in that they do not ferment sorbitol and do not exhibit glucuronidase (GUD) activity. They are also easily identified by their ability to produce Shiga toxins (Stx) and by the presence of the somatic O157 and the flagellar H7 antigens. As a result, these traits are extensively utilized in methods used to isolate and detect O157:H7 in foods. However, new variant strains of EHEC, particularly O157:H7, are being isolated more frequently from foods, animals and humans worldwide. These include sorbitol-fermenting variants, strains that exhibit GUD, non-motile, toxigenic variants; serological variants; toxin non-producing strains, etc. These variants carry most of the trait EHEC virulence markers, so are potentially pathogenic and some have already been implicated in illness; however, due to changes in trait genotypic and phenotypic markers, EHEC variants elude detection by current testing methods.

Cat# DD-0095-01-ZJ
（Ⅰ）LightCycler 1.0； (Internal Control is not included for this system)
（Ⅱ）LightCycler2.0，

Please download manual (PDF, 172 KB)

Cat# DD-0095-02-ZJ
（Ⅲ）PE5700，MJ-Opticon etc. single color systems；
（Ⅳ）ABI7000，ABI7300，ABI7500，ABI7900，ABI StepOne,StepOne plus, MJ
Opticon2，MJ-chromo4，MX3000P，MX3005P，Smart CyclerII，Rotor-Gene6000,LightCyclerR480；iCycler iQ4，iCycler iQ5, etc, multi-color systems.

Please download manual (PDF, 242 KB)

Release of MoBiTec Newsletter VIII

The new international Newsletter VIII includes:

• The new photoactivatable and photoconvertible fluorescent protein mIris FP, an advanced version of the well known EosFP.
  
• M-Beads Magnetic silica beads for diverse proteomic applications
  
• High yield CMV promoter-based constitutive expression vectors
  
• Anti-hPSTI and Anti-g3p (pIII) antibodies
  
• Highly efficient BactoLyse bacteria protein extraction reagent
  
• NeuroTrans and siRTrans transfection reagents for efficient transfection into primary neurons and transfection of siRNA into mammalian cells
  
• New plastic ware for microscopy: Imaging Dishes and ImmunoSelect^® Adhesion Slides
  
• Optimized high performance (hp) vectors for protein expression in Bacillus megaterium

Download the new newsletter as pdf (752 kb) or request a paper copy for free.

Photoactivatable and photoconvertible fluorescent protein - mIrisFP

IrisFP is a photoactivatable fluorescent protein that combines irreversible photoconversion from a green- to a red-emitting form with reversible photo-switching between a fluorescent and a non-fluorescent state in both forms. mIrisFP is a monomeric variant. mIrisFP multiple photo-activation modes can be used for pulse-chase experiments combined with subdiffraction-resolution imaging in living cells by using dual-color photo-activation localization microscopy (PALM).

Monomeric mIrisFP has excellent properties as a genetically encoded fluorescence marker. mIrisFP fluorescence can be reversibly switched on and off (useful for high-resolution microscopy, PALM), but also allows for the non-reversible photo-conversion from green to red (useful for dynamic measurements in living cells). The dual photo-activation capability of mIrisFP offers enhanced flexibility and also enables the combination of pulse-chase experiments with super-resolution imaging.

Thermostable, photoconvertible fluorescent protein – mEosFP(Thermostab)

mEosFP(Thermostab) is an advanced variant of the green to red photoconvertible fluorescent protein EosFP. The marker was optimized for functional expression at 37 °C, especially in fusion with other proteins. mEosFP(Thermostab) retains the monomeric nature of its predecessor and shows an excellent performance in fusion even with demanding partners such as tubulin. mEosFP(Thermostab) can be efficiently converted from green to red by a light pulse at wavelengths between 360 and 430 nm.

For more information please visit our photoconvertible fluorescent proteins.

Superior accessories for fluorescence microscopy and imaging

Our cell culture and microscopy ware for imaging applications provides a great opportunity, when living or fixed cells are in the focus of your research. In addition to our ImmunoSelect® Adhesion slides we are now offering plastic ware for fluorescence microscopy. The range comprises Imaging Dishes with and without µgrids and diverse Imaging Plates with different surfaces and made out of variable materials. Most products provide Tissue Culture surfaces and promise high image quality, cell adhesion and differentiation.

Until March 31 you can get a free sample of Imaging Dish CG or Adhesion slides on request. Please contact us or your local distributor.

Our whole range of fluorescence microscopy accessories can be found here.

Mobicol brochure online available

Mobicols are practical plastic tools suitable for diverse lab applications. In our new brochure you can get an overview on miscellaneous Mobicols and their applications. Different chapters describe the plastic columns and their usage:

Chapter I       Mobicol – a practical tool for every lab
Chapter II      Spin columns for your own matrices or other compounds
Chapter III     Matrix-filled MobiSpin columns for purification of nucleic acids
Chapter IV    Mobicols for affinity purification of biomolecules
Chapter V     Mobicols with enzymatically active matrices

Please download the new Mobicol brochure as pdf.

Click-Chemistry

Click-chemistry was first introduced by K. Berry Sharpless in 2001. It describes a chemical reaction which builds substances by joining small units together. Click-reactions are fast and purposeful with high yields.
The most popular click-reaction is azide alkyne Huisgen cycloaddition with Copper (Cu) as catalyst at room temperature.
Now you can use this highly efficient technology for labeling of DNA or RNA via solid-phase synthesis or PCR. Furthermore, it is possible to introduce up to three different modifications onto one position of the same DNA strand. Of course, we offer different kinds of non-fluorescent and fluorescent labels.

For more information please visit our Click-Chemistry overview.

New Vector Systems Brochure available from MoBiTec

Our new brochure focuses on gene expression in different microorganisms like Escherichia coli, Bacillus megaterium, Bacillus subtilis, Lactococcus lactis and yeast. Furthermore, it includes expression systems for mammalian cells as well as vector systems for many other purposes..

Content:

• NICE^® Expression System for Lactococcus lactis
  
• Bacillus megaterium Expression Systems
  
• Bacillus subtilis Expression Vectors
  
• pBacTag Tagging Vectors
  
• Yeast Expression Vectors
  
• pORF-CLONE Vector System
  
• pPICHOLI Shuttle Vector System
  
• Mammalian Expression Vectors
  
• CMV Expression Vectors
  
• Fusion Protein Cloning Systems
  
• BRP Plasmids and Competent BRP Cells
  
• PCR Cloning Vector p3T
  
• Poly(His)-tag Cloning Vector pEG-His1
  
• Exontrap Cloning Vector
  
• ssDNA-Production and Expression in pMEX
  
• Promoter-Trap Vector pBBR RESO
  
• Broad-Host-Range Vectors pBBR122 and pBHR1
  
• Multiple Cloning Site Vector pMCS5
  
• Standard Cloning Vectors
  
• EosFP – Green to Red Photoconvertible Fluorescent Protein
  
• Related Products

Please download the new Vector Systems Brochure (PDF, 3879 KB) !

High Yield Gene Expression with the new T7 RNA Polymerase Gene Expression System!

This system combines the benefits of the tightly regulated and strong T7 RNA polymerase gene expression system and alkaline protease free Bacillus megaterium.

The T7 RNAP gene expression system is tightly regulated and efficiently inducible through the xylA operon and the strong T7 RNAP promoter resulting in stable, high yield protein production up to 8-fold increased in comparison to common xylose inducible expression (Fig. 1).

The system is based on two parallel-replicating plasmids: pT7-RNAP and pP_T7.
In addition to the t7 rnap gene under control of the strong xylA promoter pT7-RNAP contains the genes of ampicillin and chloramphenicol for easy selection in E. coli (Amp^R) and B. megaterium (Cm^R).
pP_T7 is responsible for the T7 RNAP-dependent expression of the target gene. Downstream of the T7 promoter it comprises a multiple cloning site with ten unique restriction enzyme cleavage sites. Additionally, the plasmid carries two resistances to ampicillin (in E. coli) and tetracycline (in B. megaterium).

Fig. 1 Comparison of GFP amounts produced by variant expression systems. GFP produced by B. megaterium carrying the common xylose-regulated Plasmide pRBBm34 (square), B. megaterium transformed with the T7 expression system plasmids (circle) and B.megaterium with the T7 expression system plasmids treated with rifampicin. (Gamer et al., 2009)

Pretransformed B. megaterium protoplasts!

For your convenience we offer B. megaterium protoplasts pretransformed with pT7-RNAP, so that you just have to insert your gene of interest into pP_T7 and transform the pretransformed protoplasts with this plasmid.