Features

• Brightness- MFP dyes exhibit more brightness than the commonly used dyes
  
  
• High emission fluorescence - MFP dyes have optimal light emission due to high quantum yield values
  
  
• High fluorescence intensity - MFP dyes exhibit very intense fluorescence resulting from the high extinction coefficients and quantum yields of the dyes
  
  
• High photostability - MFP dyes are more photostable than most of the commonly used fluorescent dyes. You have more time for observation and image capture.
  
  
• High water solubility - MFP dyes are highly water soluble allowing easy protein labeling procedures without the use of organic solvents
  
  
• pH insensitivity - MFP dyes remain highly fluorescent over a broad pH range (pH 4 - 9)
  
  
• Instrument compatibility - Excitation and emission spectra of the MFP dyes are compatible with the commonly used excitation sources and filter sets

The MFP dye series

MFP488 is compatible with green emitting fluorophores such as Cy™2 and fluorescein. MFP488 is brighter than fluorescein, much more photostable, water soluble and pH insensitive over a broad range (pH 4-9).
Exc./em. maximum: 501/523 nm.

MFP555 is the perfect choice if you want to replace the red fluorescent Cy™3 dye. Antibodies labeled with this dye are optimally suited for TMR and Cy™3 filters.
Exc./em. maximum: 547/572 nm.

Figure 3: Absorption and fluorescence emission spectra of MFP488 and MFP555 dye in ethanol. Absorption and emission maxima shift to 501/523 nm for MFP488 and to 560/585 nm for MFP555 (measuring MFP488 goat anti-mouse IgG antibody and MFP555 goat anti-mouse IgG antibody in PBS buffer, pH 7.2).

MFP590 conjugates produce bright red fluorescence emission. Antibodies labeled with this dye are optimally suited for Texas Red^® filters.
Exc./em. maximum: 597/624 nm.

MFP631 can be optimally excited with the 633 nm spectral line of the He-Ne laser and the 635 nm line of red diode lasers. The bright and photostable MFP631 emitting in the far red region of the spectrum.
Exc./em. maximum: 637/658 nm.

Figure 4: Absorption and fluorescence emission spectra of MFP590 and MFP631 dye in ethanol. Absorption and emission maxima shift to 597/624 nm for MFP590 and to 633/658 nm for MFP631 (measuring MFP590 goat anti-mouse IgG antibody and MFP631 goat anti-mouse IgG antibody in PBS buffer, pH 7.2).

Labeled Streptavidin

Streptavidin is a bacterially derived biotin-binding protein that reportedly exhibits less nonspecific binding than avidin. Streptavidin, a nonglycosylated 52,800-dalton protein with a near-neutral isoelectric point, reportedly exhibits less nonspecific binding than avidin. However, streptavidin contains the tripeptide sequence Arg-Tyr-Asp (RYD) that apparently mimics the Arg-Gly-Asp (RGD) binding sequence of fibronectin. We offer streptavidin labled with MFP488.

R-Phycoerythrin is an intensely bright phycobiliprotein with absorption maxima at approximately 496, 546 and 565 nm, and a fluorescence emission maximum of ~578 nm. Allophycocyanin is an intensely bright phycobiliprotein with a broad absorption profile that peaks at ~650 nm, and a fluorescence emission maximum of ~660 nm.

Labeled R-Phycoerythrin (R-PE), see order box below.

order information

MFP Protein Labeling Kits

For efficient protein or antibody labeling we offer the ready-to-use MFP Protein Labeling Kits. These new protein labeling kits provide a convenient tool for labeling your specific antibody or desired protein with our MFP dyes:

Advantages

• Convenient - Very simple protocol
  
• Ready-to-use - All buffers and purification columns are included - no equilibration necessary
  
• Fast - Time saving procedure
  
• Quality control - Premeasured reactive dyes
  
• Advantage of commonly used protein labeling kits - Spin columns for buffer exchange and protein concentration are included!

order information

Click on the respective order # to download a detailed data sheed.

We are also offer an extending range of MFP dye labeled secondary antibodies.