products | Antibodies

Secondary Antibodies

MFP Antibodies
 

Secondary Antibodies labeled with MFP dyes

We are offering now high-quality fluorescent labeled antibodies with excellent price / performance ratio.

The newly developed MFP dyes are an ideal and cost-effective alternative to currently existing well-known fluorophores such as Cy™ labels. They can be readily used to label biomolecules (e.g. antibodies, peptides or nucleic acids) very efficiently. They are of exceptional high quality and performance.
Figure 1: The cytoskeleton in HEp2 cells has been detected using mouse monoclonal β-tubulin antibody, visualized with MFP488 goat anti-mouse (A-C) and with a well-known competitive goat anti-mouse (D-F). Nuclei were labeled using TO-PRO-3®. A,D: secondary antibody concentration 1:250; B,E: 1:250 after bleaching (100% light intensitiy for 30 seconds); C,F: 1:500.

 
 

MFP dyes have several advantages over competing brands including

  • Brightness - MFP dyes exhibit more brightness than the commonly used dyes
  • High emission fluorescence - MFP dyes have optimal light emission due to high quantum yield values
  • High fluorescence intensity - MFP dyes exhibit very intense fluorescence resulting from the high extinction coefficients and quantum yields of the dyes
  • High photostability - MFP dyes are more photostable than most of the commonly used fluorescent dyes. You have more time for observation and image capture.
  • High water solubility - MFP dyes are highly water soluble allowing easy protein labeling procedures without the use of organic solvents
  • pH insensitivity - MFP dyes remain highly fluorescent over a broad pH range (pH 4 - 9)
  • Instrument compatibility - Excitation and emission spectra of the MFP dyes are compatible with the commonly used excitation sources and filter sets



Figure 2: Relative fluorescence of MFP488 goat anti-mouse conjugates at various dye/protein ratios (excitation at 500 nm).
 

 

The MFP dye series

MFP488 is compatible with green emitting fluorophores such as Cy™2 and fluorescein. MFP488 is brighter than fluorescein, much more photostable, water soluble and pH insensitive over a broad range (pH 4-9). Exc./em.maximum: 501/523 nm.

MFP555 is the perfect choice if you want to replace the red fluorescent Cy™3 dye. Antibodies labeled with this dye are optimally suited for TMR and Cy™3 filters. Exc./em. maximum: 560/585 nm.

MFP488 MFP555
Figure 3: Absorption and fluorescence emission spectra of MFP488 and MFP555 dye in ethanol. Absorption and emission maxima shift to 501/523 nm for MFP488 and to 560/585 nm for MFP555 (measuring MFP488 goat anti-mouse IgG antibody and MFP555 goat anti-mouse IgG antibody in PBS buffer, pH 7.2).
 

MFP590 conjugates produce bright red fluorescence emission. Antibodies labeled with this dye are optimally suited for Texas Red® filters. Exc./em. maximum: 597/624 nm.

MFP631 can be optimally excited with the 633 nm spectral line of the He-Ne laser and the 635 nm line of red diode lasers. The bright and photostable MFP631 emits in the far red region of the spectrum. Exc./em. maximum: 633/658 nm.

MFP590 MFP631
Figure 4: Absorption and fluorescence emission spectra of MFP590 and MFP631 dye in ethanol. Absorption and emission maxima shift to 597/624 nm for MFP590 and to 633/658 nm for MFP631 (measuring MFP590 goat anti-mouse IgG antibody and MFP631 goat anti-mouse IgG antibody in PBS buffer, pH 7.2).

Order Information
Click on the respective order # to download a detailed data sheet.
LIST OF ANTIBODIES: ORDER INFORMATION, SHIPPING AND STORAGE
order# product unit size absorbed against
MFP-A1001 MFP488 goat anti-mouse IgG (H+L), 1 mg/ml 1 ml hu IgG and serum
MFP-A2422 MFP555 goat anti-mouse IgG (H+L), 1 mg/ml 1 ml hu IgG and serum
MFP-A1005 MFP590 goat anti-mouse IgG (H+L), 1 mg/ml 1 ml hu IgG and serum
MFP-A2050 MFP631 goat anti-mouse IgG (H+L), 1 mg/ml 1 ml hu IgG and serum
MFP-A1029 MFP488 goat anti-mouse IgG (H+L), mcr*, 1 mg/ml 1 ml bv,ch,gt,gp,ham,hs,hu,rb,rt,sh
MFP-A2424 MFP555 goat anti-mouse IgG (H+L), mcr*, 1 mg/ml 1 ml bv,ch,gt,gp,ham,hs,hu,rb,rt,sh
MFP-A1032 MFP590 goat anti-mouse IgG (H+L), mcr*, 1 mg/ml 1 ml bv,ch,gt,gp,ham,hs,hu,rb,rt,sh
MFP-A2052 MFP631 goat anti-mouse IgG (H+L), mcr*, 1 mg/ml 1 ml bv,ch,gt,gp,ham,hs,hu,rb,rt,sh
MFP-A2042 MFP488 goat anti-mouse IgM (µ chain), 1 mg/ml 0.5 ml
MFP-A2426 MFP555 goat anti-mouse IgM (µ chain), 1 mg/ml 0.5 ml
MFP-A2044 MFP590 goat anti-mouse IgM (µ chain), 1 mg/ml 0.5 ml
MFP-A2046 MFP631 goat anti-mouse IgM (µ chain), 1 mg/ml 0.5 ml
MFP-A1006 MFP488 goat anti-rat IgG (H+L), *mcr, 1 mg/ml 1 ml bv,ch,gt,gp,ham,hs,hu,ms,rb,sh
MFP-A2434 MFP555 goat anti-rat IgG (H+L), *mcr, 1 mg/ml 1 ml bv,ch,gt,gp,ham,hs,hu,ms,rb,sh
MFP-A1007 MFP590 goat anti-rat IgG (H+L), *mcr, 1 mg/ml 1 ml bv,ch,gt,gp,ham,hs,hu,ms,rb,sh
MFP-A2094 MFP631 goat anti-rat IgG (H+L), *mcr, 1 mg/ml 1 ml bv,ch,gt,gp,ham,hs,hu,ms,rb,sh
MFP-A1008 MFP488 goat anti-rabbit IgG (H+L), 1 mg/ml 1 ml hu, ms, bv
MFP-A2428 MFP555 goat anti-rabbit IgG (H+L), 1 mg/ml 1 ml hu, ms, bv
MFP-A1012 MFP590 goat anti-rabbit IgG (H+L), 1 mg/ml 1 ml hu, ms, bv
MFP-A2070 MFP631 goat anti-rabbit IgG (H+L), 1 mg/ml 1 ml hu, ms, bv
MFP-A1034 MFP488 goat anti-rabbit IgG (H+L), *mcr, 1 mg/ml 1 ml bv,ch,gt,gp,hs,hu,ms,rt,sh
MFP-A2429 MFP555 goat anti-rabbit IgG (H+L), *mcr, 1 mg/ml 1 ml bv,ch,gt,gp,hs,hu,ms,rt,sh
MFP-A1037 MFP590 goat anti-rabbit IgG (H+L), *mcr, 1 mg/ml 1 ml bv,ch,gt,gp,hs,hu,ms,rt,sh
MFP-A2071 MFP631 goat anti-rabbit IgG (H+L), *mcr, 1 mg/ml 1 ml bv,ch,gt,gp,hs,hu,ms,rt,sh
MFP-A1013 MFP488 goat anti-human IgG (H+L), *mcr, 1 mg/ml 1 ml bv,ch,gt,gp,ham,hs,ms,rb,rt,sh
MFP-A2433 MFP555 goat anti-human IgG (H+L), *mcr, 1 mg/ml 1 ml bv,ch,gt,gp,ham,hs,ms,rb,rt,sh
MFP-A1014 MFP590 goat anti-human IgG (H+L), *mcr, 1 mg/ml 1 ml bv,ch,gt,gp,ham,hs,ms,rb,rt,sh
MFP-A2091 MFP631 goat anti-human IgG (H+L), *mcr, 1 mg/ml 1 ml bv,ch,gt,gp,ham,hs,ms,rb,rt,sh
MFP-A1015 MFP488 donkey anti-sheep IgG (H+L), *mcr, 1 mg/ml 1 ml ch,gp,ham,hs,hu,ms,rb,rt
MFP-A2436 MFP555 donkey anti-sheep IgG (H+L), *mcr, 1 mg/ml 1 ml ch,gp,ham,hs,hu,ms,rb,rt
MFP-A1016 MFP590 donkey anti-sheep IgG (H+L), *mcr, 1 mg/ml 1 ml ch,gp,ham,hs,hu,ms,rb,rt
MFP-A2100 MFP631 donkey anti-sheep IgG (H+L), *mcr, 1 mg/ml 1 ml ch,gp,ham,hs,hu,ms,rb,rt
MFP-A1055 MFP488 donkey anti-goat IgG (H+L), 1 mg/ml 1 ml hu,ms,rb,rt
MFP-A2432 MFP555 donkey anti-goat IgG (H+L), 1 mg/ml 1 ml hu,ms,rb,rt
MFP-A1058 MFP590 donkey anti-goat IgG (H+L), 1 mg/ml 1 ml hu,ms,rb,rt
MFP-A2082 MFP631 donkey anti-goat IgG (H+L), 1 mg/ml 1 ml hu,ms,rb,rt
MFP-A1078 MFP488 rabbit anti-goat IgG (H+L), 1 mg/ml 1 ml hu
MFP-A2431 MFP555 rabbit anti-goat IgG (H+L), 1 mg/ml 1 ml hu
MFP-A1080 MFP590 rabbit anti-goat IgG (H+L), 1 mg/ml 1 ml hu
MFP-A2086 MFP631 rabbit anti-goat IgG (H+L), 1 mg/ml 1 ml hu
MFP-A1073 MFP488 goat anti-guinea pig IgG (H+L), *mcr, 1 mg/ml 1 ml bv,ch,gt,ham,hs,hu,ms,rb,rt,sh
MFP-A2435 MFP555 goat anti-guinea pig IgG (H+L), *mcr, 1 mg/ml 1 ml bv,ch,gt,ham,hs,hu,ms,rb,rt,sh
MFP-A1076 MFP590 goat anti-guinea pig IgG (H+L), *mcr, 1 mg/ml 1 ml bv,ch,gt,ham,hs,hu,ms,rb,rt,sh
MFP-A2105 MFP631 goat anti-guinea pig IgG (H+L), *mcr, 1 mg/ml 1 ml bv,ch,gt,ham,hs,hu,ms,rb,rt,sh
MFP-A2206 MFP488 donkey anti-rabbit IgG (H+L), *mcr, 1 mg/ml 1 ml bv,ch,gt,gp,hs,hu,ms,rt,sh
MFP-A3572 MFP555 donkey anti-rabbit IgG (H+L), *mcr, 1 mg/ml 1 ml bv,ch,gt,gp,hs,hu,ms,rt,sh
MFP-A2207 MFP590 donkey anti-rabbit IgG (H+L), *mcr, 1 mg/ml 1 ml bv,ch,gt,gp,hs,hu,ms,rt,sh
MFP-A3569 MFP631 donkey anti-rabbit IgG (H+L), *mcr, 1 mg/ml 1 ml bv,ch,gt,gp,hs,hu,ms,rt,sh
MFP-A2441 MFP488 chicken anti-rabbit IgG (H+L), 1 mg/ml 1 ml
MFP-A2440 MFP555 chicken anti-rabbit IgG (H+L), 1 mg/ml 1 ml
MFP-A2442 MFP590 chicken anti-rabbit IgG (H+L), 1 mg/ml 1 ml
MFP-A2444 MFP631 chicken anti-rabbit IgG (H+L), 1 mg/ml 1 ml
shipped on blue ice; store at -20°C
(once thawed, store at 4°C to avoid multiple freezing and thawing)
Abbreviations:
bv - bovine, ch - chicken, gp - guinea pig, gt, - goat, ham - hamster, hs - horse, hu - human, ms - mouse, rb - rabbit, rt - rat, sh - sheep,

Texas Red and TO-PRO-3 is registered trademark of Molecular Probes, Cy is trademark of Amersham Bioscience

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