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MobiTEV Protease |
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Improved TEV Protease at an Affordable Price
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TEV Protease is used to remove fusion tags - solubility, secretion, detection, and purification tags - and release the "native" protein of interest. MobiTEV Protease is an improved version of Tobacco Etch Virus (TEV) protease that is highly site-specific, highly active, and significantly more stable than native TEV Protease, resulting in enhanced long-term activity. MobiTEV Protease specifically recognizes a seven amino acid sequence (Glu-Asn-Leu-Tyr-Phe-Gln-Gly, cleaving between Gln and Gly). |
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Features
- Highly specific cleavage activity
- Robust activity over a broad temperature (4 °C to 37 °C) and pH (7 to 9) range
- Enhanced enzyme stability for prolonged protease activity
- Greater than 85% single-band purity with no non-specific protease contamination
- Easy removal of MobiTEV Protease from the digested protein sample using the polyhistidine
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Unit Definition
One unit of MobiTEV Protease cleaves 3 µg of the control substrate at >85% in 1 hour in 30 µl reaction buffer (50 mM Tris/HCl, 0.5 mM EDTA and 1mM DTT) at pH 8.0 and 30 °C. |
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Formulation and Storage
- 1000 U MobiTEV Protease (2 U/µl):
is formulated in 50 mM Tris/HCl pH 7.5, 1 mM EDTA, 5 mM DTT, 70 mM imidazol, 50% (v/v) glycerol.
Store at -80 °C. Avoid repeated freeze-thaw cycles. Stable for 6 months when properly stored.
- 1 ml 20x MobiTEV Reaction Buffer, Tris-HCl pH 8.0, 10 mM EDTA
Store at 4 °C
- 500 µl 0.1 M DTT in deionized water
Store at -20 °C
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References
- Kapust et al. (2001) Tobacco etch virus protease: mechanism of autolysis and rational design of stable mutants with wild-type catalytic proficiency. Protein Eng. 14, 993-1000.
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Order Information
Please click on the respective order # for further details. |
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| Order # |
Description |
Amount |
| PR-ETA10010-00* |
MobiTEV Protease, recombinant, His-tag |
40 U |
| PR-ETA10010-01* |
MobiTEV Protease, recombinant, His-tag |
1000 U |
| PR-ETA10010-05* |
MobiTEV Protease, recombinant, His-tag |
10 x 1000 U |
Storage: Enzyme at -20 °C, long-term storage at -80 °C; Buffer at 4 °C; DTT at -20 °C;
* not available in the US & Canada |
Related Products: TEV Protease with additional GST-tag
Please click on the respective order # for further details.
| Order # |
Description |
Amount |
| PR-ETA10050-01* |
TEV Protease (TurboTEV), recombinant, GST- & His-tag |
10 µg (100 U) |
| PR-ETA10050-02* |
TEV Protease (TurboTEV), recombinant, GST- & His-tag |
50 µg (500 U) |
| PR-ETA10050-03* |
TEV Protease (TurboTEV), recombinant, GST- & His-tag |
100 µg (1.000 U) |
| PR-ETA10050-04* |
TEV Protease (TurboTEV), recombinant, GST- & His-tag |
1 mg (10.000 U) |
| Storage: Enzyme at -20 °C; * not available in the USA & Canada |
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