news

MoBiTec - Product Highlights - NEWS
  14.07.2010 MoBiTec      
 

Newsletter VII available from MoBiTec

The Newsletter VII with products from MoBiTec and partners is just released, including:

  • T7 RNA Polymerase Gene Expression System for Bacillus megaterium
  • RetroNectin® - Retroviral mediated transfection as alternative to chemical mediated transfection
  • K-2500s PIP3 Mass ELISA Kit – non radioactive detection of PIP3
  • LIVE/DEAD® Viability Kits – wide range of viability assays
  • DNAgard™ – Store and ship DNA at room temperature
  • TransIT® - 2020 – High performance DNA transfection reagent
  • Z-Fish™ Antibodies for zebrafish research
  • IL-18 ELISA Kit – measuring of interleukin 18

Download the new newsletter as pdf (1008 kb) or request a paper copy for free.

 

   MoBiTec Newsletter V  
         
  28.05.2010 MoBiTec      
 

Click-Chemistry

Click-chemistry was first introduced by K. Berry Sharpless in 2001. It describes a chemical reaction which builds substances by joining small units together. Click-reactions are fast and purposeful with high yields.
The most popular click-reaction is azide alkyne Huisgen cycloaddition with Copper (Cu) as catalyst at room temperature.
Now you can use this highly efficient technology for labeling of DNA or RNA via solid-phase synthesis or PCR. Furthermore, it is possible to introduce up to three different modifications onto one position of the same DNA strand. Of course, we offer different kinds of non-fluorescent and fluorescent labels.

For more information please visit our Click-Chemistry overview.



  





 
  11.05.2010 Takara      
 

SapphireAmp® Fast PCR Mix

Features:

  • Faster than standard Taq: reactions completed in1/2 the time of conventional Taq
  • Restriction enzyme digestion can be directly performed on PCR products in the PCR buffer
  • PCR reaction can be directly loaded onto a gel without purification or addition of other reagents
  • Save time and eliminate purification steps with no reduction in yield

 

Description:

Fig.: Template: Human Genome 100ng/50mL Targets: 1: p53 0.5 kb (54%); 2: FFAR2 1.0 kb (59%); 3: DCLRE1A 2.0 kb (38%); 4: p53 4.2 kb (48%); 5: IGF2R 5.9 kb (50%)

SapphireAmp® Fast PCR Master Mix contains a hot start PCR enzyme, optimized buffer, dNTP mixture, gel loading dye (blue) and a density reagent in a 2X premix. PCR reactions with this mix can be completed in half the time required for conventional Taq amplifications. Amplification of human genomic DNA targets of 2 kb in length can be completed in 1 hour, and amplifications of human genomic DNA targets of up to 6 kb are possible using Sapphire-Amp™. This product is well-suited for E. coli-based colony PCR to check for inserts of up to ~5 kb, and colony checks can be completed in about 1 hour. SapphireAmp® Fast PCR Master Mix reactions can be assembled by simply adding primer and DNA template to the premix. Reactions performed with this mix can be loaded directly onto a gel for electrophoresis, and restriction digestion of PCR products is possible in SapphireAmp™ reaction buffer. SapphireAmp® Fast PCR Master Mix offers a powerful, fast, flexible and convenient alternative for both simple and complex PCR applications.

Table: SapphireAmp® Master Mix saves time.

Length TaKaRa SapphireAmp®
Fast PCR Master Mix		 Company P Company T
0.5 kb 50 min
 1 hr 35 min
 1 hr 30 min
1 kb 55 min 1 hr 50 min 1 hr 45 min
2 kb 1 hr 2 hr 20 min 2 hr 15 min
4 kb 1 hr 30 min 3 hr 20 min 3 hr 15 min
4 kb 2 hr 4 hr 20 min 4 hr 15 min

 

Order Information:

Order Information
order # description amount 
RR350A-TK SapphireAmp® Fast PCR Master Mix 160 reactions 


 





 
  03.03.2010 MoBiTec      
 

New Vector Systems Brochure available from MoBiTec

Our new brochure focuses on gene expression in different microorganisms like Escherichia coli, Bacillus megaterium, Bacillus subtilis, Lactococcus lactis and yeast. Furthermore, it includes expression systems for mammalian cells as well as vector systems for many other purposes.

Content:

  • NICE® Expression System for Lactococcus lactis
  • Bacillus megaterium Expression Systems
  • Bacillus subtilis Expression Vectors
  • pBacTag Tagging Vectors
  • Yeast Expression Vectors
  • pORF-CLONE Vector System
  • pPICHOLI Shuttle Vector System
  • Mammalian Expression Vectors
  • CMV Expression Vectors
  • Fusion Protein Cloning Systems
  • BRP Plasmids and Competent BRP Cells
  • PCR Cloning Vector p3T
  • Poly(His)-tag Cloning Vector pEG-His1
  • Exontrap Cloning Vector
  • ssDNA-Production and Expression in pMEX
  • Promoter-Trap Vector pBBR RESO
  • Broad-Host-Range Vectors pBBR122 and pBHR1
  • Suicide Vector pCorrectClone
  • Multiple Cloning Site Vector pMCS5
  • Standard Cloning Vectors
  • EosFP – Green to Red Photoconvertible Fluorescent Protein
  • Related Products

Please download the new Vector Systems Brochure (PDF, 3879 KB) !

  





 
  18.02.2010 Amresco      
 

AMRESCO’s InvestiGator including products for:

  • Protein Electrophoresis
  • Western Blotting
  • Proteomics


Download the new Newsletter as PDF (1600KB) or request a paper copy for free.

  


 
  29.01.2010 MoBiTec      
 

High Yield Gene Expression with the new T7 RNA Polymerase Gene Expression System!

This system combines the benefits of the tightly regulated and strong T7 RNA polymerase gene expression system and alkaline protease free Bacillus megaterium.

The T7 RNAP gene expression system is tightly regulated and efficiently inducible through the xylA operon and the strong T7 RNAP promoter resulting in stable, high yield protein production up to 8-fold increased in comparison to common xylose inducible expression (Fig. 1).

The system is based on two parallel-replicating plasmids: pT7-RNAP and pPT7.
In addition to the t7 rnap gene under control of the strong xylA promoter pT7-RNAP contains the genes of ampicillin and chloramphenicol for easy selection in E. coli (AmpR) and B. megaterium (CmR).
pPT7 is responsible for the T7 RNAP-dependent expression of the target gene. Downstream of the T7 promoter it comprises a multiple cloning site with ten unique restriction enzyme cleavage sites. Additionally, the plasmid carries two resistances to ampicillin (in E. coli) and tetracycline (in B. megaterium).

Fig. 1 Comparison of GFP amounts produced by variant expression systems. GFP produced by B. megaterium carrying the common xylose-regulated Plasmide pRBBm34 (square), B. megaterium transformed with the T7 expression system plasmids (circle) and B.megaterium with the T7 expression system plasmids treated with rifampicin. (Gamer et al., 2009)

Pretransformed B. megaterium protoplasts!

For your convenience we offer B. megaterium protoplasts pretransformed with pT7-RNAP, so that you just have to insert your gene of interest into pPT7 and transform the pretransformed protoplasts with this plasmid.


Order Information, Shipping & Storage
order # description amount 
BMEGT702 Bacillus megaterium protoplasts, strain MS941, pretransformed with pT7-RNAP 5 x 500 µl 
BMEGT701 Bacillus megaterium high yield T7 gene expression kit, includes pretransformed protoplasts BMEGT702 (5 x 500µl), pPT7 cloning vector and pPT7-GFP control vector (vectors lyophilized, 10 µg each)
kit 
BMEGT710 Bacillus megaterium pPT7 cloning vector, lyophilized 10 µg 


     
  21.01.2010 Molecular Probes      
 

CellTrace™ Violet Cell Proliferation Kit (C34557)

The new gold standard in cell proliferation assays featuring CellTrace™ Violet

The class of fluorescent compounds known as cell tracking dyes is fundamental to our current understanding of the immune system and plays a major role in cancer, HIV, and post-transplant research. Several types of dyes fall into this category: some that hydrophobically insert into the plasma membrane, some which react with intracellular thiols, and others which covalently bind to amine groups inside cells. Amine-reactive cell tracking dyes, such as the new CellTrace™ Violet, are known to provide excellent long-term cell tracing. Initially a non-fluorescent ester of a fluorescent molecule, CellTrace™ Violet passively enters cells where it is converted to a fluorescent derivative by nonspecific esterases. The succinimidyl ester then covalently binds to amine groups in proteins, resulting in long-term dye retention.
When a CellTrace™ Violet-labeled cell divides, each daughter cell receives approximately half of the fluorescent label, the next generation receives one-fourth, and so on. Analysis of the fluorescence intensities of cells labeled and grown in vivo permits the determination of the number of generations through which a cell has progressed since the label was applied. This tool allows the researcher to look for dividing cells in biological systems.
The violet excitation and blue emission of CellTrace Violet will allow the researcher to perform cell proliferation experiments with a wide range of blue-excitable fluorophores, including GFP-expressing cells.

Order Information
order # description amount 
AZC34557 CellTrace™ Violet Cell Proliferation Kit kit 


     
 

Absolute Detection of Apoptotic Transformation
- Violet Ratiometric Membrane Asymmetry Probe/Dead Cell Apoptosis Kit

What it is

The Violet Ratiometric Membrane Asymmetry Probe/Dead Cell Apoptosis Kit provides an easy, efficient method for the detection of apoptosis using a violet laser flow cytometer. The assay is compatible with adherent and suspension cell types and correlates with other indicators of apoptosis, including caspase activation and changes in mitochondrial membrane potential.

What it offers

  • Robust assay - ratiometric probe is independent of probe concentration, cell size, and instrument variation
  • Easy to use - 5-minute incubation, with no wash required
  • Flexible experimental design - easily combined with other testing reagents

How it works

This assay employs a novel dye (F2N12S) that incorporates selectively into the outer leaflet of the plasma membrane. The dye exhibits dual fluorescence emission bands corresponding to 530 nm and 585 nm, producing a ratiometric response to variations in plasma membrane surface charge during early apoptosis that is detected by flow cytometry. The kit also includes SYTOX® AADvanced™ Dead Cell Stain for discrimination of dead cells from live cells. Unlike annexin V conjugates, this assay requires no special buffers or wash steps and is less affected by cell membrane damage commonly associated with the removal of adherent cells, thereby improving data quality.



Order Information
order # description amount 
A35137 Violet Ratiometric Membrane AsymmetryProbe/Dead Cell Apoptosis Kit for flow cytometry 1 kit 


     
  15.12.2009 Biomatrica      
 

DNAgard™ for DNA stabilization in tissues and cells

Biomatrica introduces the DNAgard™ a new product for DNA stabilization in tissues and cells. DNAgard™ stabilizes genomic DNA in cultured cells or animal tissues for at least 2 months in liquid storage format at room temperature. For longer term storage dry-down is required.

DNAgard™ is designed for immediate stabilization of DNA from intact cells and tissues with the convenience of room temperature shipping, processing and storage. The liquid storage reagent rapidly permeates cell membranes to stabilize and protect genomic DNA.

With its excellent properties DNAgard™ is ideal for field collection and tissue harvesting. Of course DNA protected using DNAgard™ is suitable for use in many downstream applications, including long-range PCR and Real Time PCR.

For longer storage, samples can be dried down and stored at room temperature for at least 1 year* (*accelerated aging). Dry samples can be easily recovered by simple rehydration and are ready for DNA extraction

Order Information
order # description
63020-016 Trial kit: (3) tubes (500µl) of DNAgard
62001-036 DNAgard Tissues & Cells, 50 ml: DNAgard solution 50 ml
(100 x 500 μl reactions)
62001-046 DNAgard Tissues & Cells, 100 ml: DNAgard solution 100 ml
(200 x 500 μl reactions)
93026-090 Barcoded 2 ml Tubes (empty) (100) barcoded 2 ml screw cap tubes
93027-290 Barcoded DNAgard tubes (empty) (25) barcoded DNAgard tubes, (2) moisture barrier bags, (2) dessicant packets
90028-290 Barcoded 96 well-plates (empty) (5) barcoded 96 well-plates, (5) moisture barrier bags, (10) dessicant packets, (5) plate seal

 

      
  10.12.2009 Echelon Biosciences      
 

K-2500s PIP3 Mass ELISA Kit

Echelon Biosciences introduces the K-2500s PIP3 Mass ELISA. This kit is 10x more sensitive to PIP3 than our K-2500 assay and offers less cross reactivity with PIP's (most specifically PI(3,4)P2).

K-2500s - PIP3 Mass ELISA

The K-2500s uses different PIP3 Detection protein and buffers than the
K-2500 PIP3 Mass Elisa Kit, resulting in 10x more sensitivity and selectivity to PIP3.

Class I Phosphoinositide 3-kinases (PI3-Kinase, PI3-K), are ubiquitously expressed lipid kinases which phosphorylate PtdIns(4,5)P2 at the 3'-hydroxyl of the inositol ring producing PtdIns(3,4,5)P3 (PIP3). PIP3 has shown to be an important lipid second messenger in multiple cell signalling pathways such as proliferation, metabolism, calcium release, and survival. Due to these interesting biological roles, PI3-K and PIP3 have become attractive targets for research and drug development.

The PIP3 Mass ELISA Kit allows the using to determine PI3-K activity by quantifying the amount of PIP3 found in cells. After following a non-radioactive lipid extraction protocol, the PIP3 samples obtained from millions of cells are detected on a competitive ELISA. The cellular PIP3 samples are mixed and incubated with a PIP3 Detector protein, then added to a PIP3-coated microplate for competitive binding. A peroxidase-linked secondary detector and colorimetric detection is used to detect the PIP3 protein binding to the plate. The colorimetric signal is inversely proportional to the amount of PIP3 obtained.

Order Information
order # description
K2500s PIP3 Mass ELISA

 

      
         
  02.12.2009 MoBiTec      
 

Newsletter VI released

New Product Highlights from MoBiTec and Partners

The new MoBiTec Newsletter is just released, including:

  • Fully Food Grade Expression System for Lactococcus lactis
  • Immobilized Alkaline Phosphatase (CIP)
  • Improved TEV Protease at an Affordable Price
  • Now available - the Mobicol "F"
  • Magnetic Beads for Antibody Purification
  • MFP Antibodies
  • Cell Viability and Cytotoxicity Assay
  • Y2H Transactivating Protein System
  • New Cold-Inducible Vectors
  • AquaGenomic™

Download the new newsletter as pdf (810 kb) or request a paper copy for free.

 

   MoBiTec Newsletter V  
         
  26.11.2009 AnaSpec      
 

SensoLyteTM520 Calpain Assay
– Long Wavelength Kit

AnaSpec is pleased to announce the release of the industry’s first longest wavelength FRET based calpain assay, the SensoLyte® 520 Calpain Activity Assay Kit. Optimized for detecting calpain activity, this kit contains a novel internally quenched 5-FAM/QXL™ 520 FRET substrate. Cleavage of this FRET substrate generates two separate fragments. The subsequent release of 5-FAM fluorescence can be monitored at excitation/emission= 490/520 nm. Increase in fluorescence is proportional to the calpain activity. The assay can detect both calpain 1 (µ) and 2 (m) activities and is ideal for kinetic study of these enzymes. The long wavelength fluorescence of 5-FAM is less interfered by the autofluorescence of components in biological samples and test compounds.

Calpains are a family of intracellular cysteine proteases, which consists of at least 15 ubiquitous and tissue-specific isoforms.1 The best-characterized calpains are the isoforms µ- and m-calpain, which are also known as calpain 1 and calpain 2, respectively. Calpains are calcium-dependent and function by modulating the biological activities of many proteins through selective cleavage.2 Studies have demonstrated that calpains are implicated in a variety of calcium-regulated cellular processes such as signal transduction, cell proliferation, differentiation, cell cycle progression, apoptosis, and platelet activation. Deregulation of calpains activities has been implicated in various pathological phenomena such as atherosclerosis, Alzheimer’s disease, diabetes, and cancer.3, 4 Calpains represent potential therapeutic targets for drug development.5, 6
An AMC based calpain assay, the SensoLyte® AMC Calpain Assay, is also available from AnaSpec.

  SensoLyte® 520 Calpain Assay
(order# 72149)		 SensoLyte® AMC Calpain Assay
(order# 72150)
Read-out Fluorimetric, FRET based (5-FAM/QXL™ 520) Fluorimetric
Wavelengths Ex/Em= 490/520 nm Ex/Em= 354/442 nm
Fluorescence Green Blue
Lowest Detection Limit 0.04 µM 0.25 µM
Kit size (96-well plate) 100 tests 100 tests
Assay time As short as 1h As short as 1h
Inhibitor screening


Order Information
order # description
72149 SensoLyte® 520 Calpain Activity Assay Kit *Fluorimetric New!
72150 SensoLyte® AMC Calpain Activity Assay Kit *Fluorimetric New!

References:
1. Goll, DE. et al. Physiol. Rev. 83, 731 (2003).
2. Suzuki K, et al. Diabetes. 53 Suppl 1, S12 (2004).
3. Zatz M, et al: N Engl J Med. 352, 2413 (2005).
4. Branca D. Biochem Biophys Res Commun. 322, 1098 (2004).
5. Carragher NO. Curr Pharm Des. 12, 615 (2006).
6. Saez ME. et al. Drug Discovery Today 11, 917 (2006)

 

  
 
  06.11.2009 AnaSpec      
 

Antibody catalog 2010

AnaSpec presents its new Antibody catalog 2010, featuring antibodies for apoptosis, cancer research, neurobiology, signal transduction, as well as secondary and Z-Fish™ antibodies.

Please use this link to download your copy of it (PDF, 4.8 MB). Inquiries for specific data sheets are welcome.

  
TaKaRa catalog 2009		  
         
  07.08.2009 MoBiTec      
 

Inositol Phosphates and Phosphoinositides

Inositol phosphates play important roles in diverse cellular functions, such as cell growth, apoptosis, cell migration, endocytosis, and cell differentiation. The group of inositol phosphates includes inositol monophosphate, inositol trisphosphate, and inositol pentakisphosphate. MoBiTec offers a hugh range of Inositole Phosphates, membrane permeant Inositol Phosphates, caged Inositol Phosphates and Phosphoinositides.

Read more...

    
         
  16.07.2009 MoBiTec      
 

Macromolecular Crystallography

Macromolecular Crystallography is a technique used to study biological molecules such as proteins, to a resolution higher than ~0.5 nm (~ 5 Å). This high resolution helps elucidate the complex mechanism by which these macromolecules carry out their functions in living cells and organisms. MoBiTec now offers Screens, plates and more: all you need for crystallization of biological macromolecules.
Read more...

Download the Macromolecular Crystallography brochure, to get an overview about the available products. (PDF 2MB)

    
         
  28.05.2009 Biomatrica      
 

Stabilize your biological sample at room temperature.

Biomatrica stabilizes your samples and research. Our core technology stabilizes biological materials at room temperature – preserving sample integrity and increasing reproducibility and reliability. Our technology platform and continuous innovation enables you to perform your research with increased confidence and efficiency.

DNAstable® - Preserve and store genomic and plasmid DNA at room temperature. (PDF)
RNAstable

RNAstable™ - Preserve and Store RNA at Room Temperature.
Clonestable

CloneStable™ - Preserve and Store Bacterial DNA

PCRboost

PCRboost™ - Improve your PCR Performance

 

   Biomatrica  
         
  27.04.2009 TaKaRa      
 

TaKaRa Life Science Catalog 2009-2010 available

The brand new TaKaRa Life Science Catalog 2009-10 is now available. It includes

  • New Product Highlights
  • PCR
  • Molecular Biology
  • Cell Biology
  • Protein Research
  • Glycobiology
  • Instrumentation

Request your free paper copy today.

   TaKaRa catalog 2009  
         
  15.04.2009 Molecular Probes      
 

BioProbes 58 available

Get the latest news from Molecular Probes

COVER STORY
For a reliable and reproducible TUNEL imaging assay, it is vital not only that the modified nucleotide is an acceptable substrate for TdT, but also that the detection method is sensitive and avoids any additional loss of cells from the sample. The Click-iT® TUNEL Imaging Assays meet both of these criteria.

Download BioProbes 58 (PDF, 2.2 MB) or order a free paper copy!

 

   BioProbes 58 Cover  
         
  07.04.2009 MoBiTec      
 

Newsletter V released

New Product Highlights from MoBiTec and Partners

The new MoBiTec Newsletter is just released, including:

  • Easy-to-use EIA Kits from TaKaRa
  • Histone deacetylase (HDAC) Assay kits
  • First Class Dyes: Violet Laser FACS® Stains
  • Fully food grade Expression System for Lactococcus lactis: NICE
  • Everyday dyes at a very competitive price!
  • New storage technology for your nucleic acids
  • Fluorescent Ubiquitination-based Cell Cycle Indicator
  • Edge Performa® DTR
  • IP7 – Phosphorylation without ATP!
  • Dark Reader™ and GR Safe II – the perfect team for your nucleic acid detection

Download the new newsletter as pdf (866 kb) or request a paper copy for free.

 

   MoBiTec Newsletter V  
         
  11.03.2009 ZJ Biotech      
 

New Partner company ZJ Bio-Tech

MoBiTec anounces a new cooperation with Shanghai Zhijiang Biotechnology Co., Ltd.

Shanghai Zhijiang Biotechnology Co., Ltd (ZJ Bio-Tech), founded in 2001, is a leading biotechnology company in the diagnostic reagent field. They are specialized in development, manufacture, and sales of real time PCR detection kits. The wide range of real time PCR kits is well used in clinical diagnostics, public health incidence control, entry-exit inspection and quarantine, food safety inspection, husbandry and fishery disease detection, etc. ZJ Bio-Tech's production basis is approved with GMP certificate and part of their products have gained state patents and China FDA certificates.

Some product highlights

Download the ZJ-Bio flyer (PDF, 337 kb)

Internet: http://www.liferiver.com.cn/en

    
         
  03.02.2009 MoBiTec      
 

GR Safe Nucleic Acid Stain

More Sensitive but much less mutagen than Ethidium Bromide

GR Safe is a new, safe nucleic acid stain for detecting double-stranded DNA, single-stranded DNA, and RNA in agarose gel. It can be used for replacing mutagenic ethidium bromide (EB). GR Safe emits green fluorescence when bound to dsDNA and red fluorescence when bound to ssDNA or RNA. This new stain has two fluorescence excitation maxima when bound to nucleic acid, one centered at approximately 290 nm and one at approximately 490 nm. GR Safe is as sensitive as SYBR Green or Gelstar but much cheeper. You can use GR Safe just as the way you used EB.

Features:

  • Stains RNA, DNA and oligonucleotides (Range from 10 bp to 100 kb)
  • Ultrasensitive: detects DNA at picogram level
  • Works perfect with either UV or safer blue light transilluminator (e.g. Dark Reader®)
  • No special gel documentation system required
  • Compatible with all gel purification kits tested, will not inhibit ligation reaction etc.

Download the GR Safe flyer (PDF, 409 kb)

Order Information
order # description amount
FT-GES00200-00 GR Safe Nucleic acid gel stain, 10‘000x concentrate, Sample Size 25 μl
FT-GES00200-01 GR Safe Nucleic acid gel stain, 10‘000x concentrate 500 μl

 

    
         
  05.01.2009 MoBiTec      
 

NICE® Expression System for Lactococcus lactis

Controlled gene expression in L. lactis – an emerging alternative for recombinant protein production

Next to the wealth of traditional food applications, L. lactis is increasingly used for modern biotechnological applications such as the production of recombinant proteins for food, feed, pharma and biocatalysis applications. In addition to to the easy genetic accessibility of L. lactis the Nisin Controlled gene Expression (NICE®) system has played a key role in this development.

Features

  • The system is fully food grade
  • No endotoxins are produced
  • No inclusion bodies
  • No spores
  • No extracellular proteinases
  • Tightly controlled gene expression allows production of toxic proteins
  • Simple fermentation, scale-up and down stream processing

Read more about the new NICE® Expression System for Lactococcus lactis

   NICE Expression System for Lactococcus lactis  
         
  24.12.2008 MoBiTec      
 

MoBiTec wishes all customers Merry Christmas
and a happy & prosperous new year!

  
  22.09.2008 AnaSpec      
 

TBTA - Click Chemistry Ligand for Cu (I)

      
 

Copper (I) species are powerful catalysts for the formation of 1,2,3-triazoles from azides and alkynes. The general thermodynamic instability of Cu(I) however, results in easy oxidation to Cu(II) and/or disproportionation to Cu(0) and Cu(II).1 TBTA, Tris-[(1-benzyl-1H-1,2,3-triazol-4-yl)methyl]amine, also known as tris-(benzyltriazolylmethyl)amine, is a stabilizing ligand for Cu(I) developed by Tim Chan in Sharpless’ lab. TBTA protects Cu(I) from oxidation and dispropotionation, while enhancing its catalytic activity.1-2 A C3-symmetric derivative, TBTS is an extremely useful ligand for azide-alkyne cycloaddition with ~106 fold of rate acceleration. Its tetradentate binding ability is able to completely envelop the Cu(I) center, leaving no free binding sites available for potential destabilizing interaction. Reactions are typically carried out with 1-2 mol % Cu(I) and TBTA without having to exclude oxygen and water.3 For use as a tool in the drug discovery process, AnaSpec carries TBTA, the click chemistry reagent used in Cu(I)TBTA-catalyzed azide-alkyne cycloaddition. TBTA, with a MW of 530.6, is supplied in powder form at 97% purity.

References:
1. Chan, TR. et al. Org. Lett. 6, 2853 (2004).
2. Sharpless, KB. and MG. Finn, J. Am. Chem. Soc. 2003, 125, 3192.
3. Kolb, HC. and KB. Sharpless, Drug Discovery Today, 2003, 8, 1128.

# Order Product
Amount
63360-50AS TBTA, Tris[(1 - benzyl - 1H - 1,2,3 - triazol - 4 - yl)methyl] amine
50 mg
63360-500AS TBTA, Tris[(1 - benzyl - 1H - 1,2,3 - triazol - 4 - yl)methyl] amine
500 mg

 

  
 
  16.07.2008 MoBiTec      
 

New Endoproteases Brochure available - Including MobiTEV protease

  
 

The new brochure includes our expanding collection of Endoproteases:

Download the new Endoprotease Brochure as PDF (460 kb) or order a free paper Copy!

 

 
  02.06.2008 MoBiTec      
 

EosFP - Green to Red Photoconvertible Fluorescent Protein for Local Marking

  
  Our portfolio of fluorescent proteins has been extended by a new fluorescent protein that allows UV/blue-inducible, permanent, bright and fast photoconversion from green to red wave length. These properties make it an excellent choice as a marker for tracking of cells, compartments or proteins in live cells, as well as a nanoscopy marker using photoactivated localization microscopy (PALM).
Read more

Download our new EosFP Flyer (PDF, 317 kb) or order a free paper Copy!.
    
         
  26.05.2008 MoBiTec      
 

New brochure on Molecular Screening Technology available from MoBiTec

  
 

This 16-page brochure focuses on several yeast one- and two-hybrid systems and a phagemid system, that are useful for high-throughput molecular screening of protein-protein and DNA-protein interactions. Further tools, such as biochemical assays, immunological and fluorescence-based methods, are available from MoBiTec as well. The yeast two-hybrid system has rapidly become one of the most widely used techniques in molecular biology. It still is the method of choice to identify protein-protein interactions from either cDNA libraries or known gene sequences in vivo. The method relies on the transactivation of reporter genes in Saccharomyces cerevisiae identifying positive interactions.

Download the new Molecular Screening Brochure (1.2 MB) or order a free paper Copy!

Content:

Grow’n’Glow GFP Reporter Systems

MoBiTec’s Grow’n’Glow one- and two- hybrid systems are powerful tools for gene discovery and functional analysis. A GFP reporter allows one-step selection of positive clones under UV light.

Y2H Membrane Systems

MoBiTec’s split-ubiquitin based systems circumvent the restrictions that are usually associated with traditional Y2H systems, now allowing to detect interactors with integral membrane proteins, self-activating and transcriptionally active proteins.

  • Y2H Membrane System
    Versatile, reliable detection and analysis of specific binding partners of full-length integral membrane and membrane-associated proteins.
  • Y2H Transactivating Protein System
    Screens transcriptionally active and self-activating proteins that cannot be screened using classical Y2H systems.

Tools and Media

MoBiTec offers many tools optimized for working with Y2H and yeast: Kits to transform yeast cells, kits to purify plasmids from yeast, media, supplements and fermentation technology.

Phagemid System

  • Phagemid Display System
    Easy-to-handle, fast and low-cost approach for highthroughput and high-content screening of protein-protein interactions.

Download the new Molecular Screening Brochure (1.2 MB) or order a free paper Copy!

 

 
         
  13.05.2008 Molecular Probes      
 

BioProbes 56 available

      
 

Get the latest information on cell biology products from Molecular Probes and their applications. Download the entire BioProbes 56 newsletter (PDF, 6.82 MB) or view individual articles below.

 
 
         
  28.04.2008 LifeSpan      
 

New Partner Company - LifeSpan

      
 

MoBiTec is proud to announce the appointment of a leading antibody manufacturer in molecular pathology - LifeSpan Biosciences. LifeSpan offers one the industry's largest inventory of rabbit polyclonal anti-human peptide antibodies to gene families including the G Protein-Coupled Receptors (GPCRs), Nuclear Receptors (NRs), and Kinases. LifeSpan's antibodies have been especially optimized for immunohistochemistry applications in human tissues.

Please use our product search for the product range of more than 30'000 antibodies from LifeSpan.

About LifeSpan BioSciences, Inc.

LifeSpan BioSciences, headquartered in Seattle, Washington, is a privately held company founded in 1995 that employs human tissues, specific antibodies and immunohistochemistry to identify and validate targets for its biotechnology and pharmaceutical company customers in Europe, Asia and North America. Products and services include more than 1,300 antibodies, custom IHC-validated antibody synthesis, IHC contract research services, custom tissue microarray (TMA) construction for use in contract research studies, pathology and imaging services, monoclonal antibody cross-reactivity screening services, subscription access to comprehensive human tissue IHC localization profiles for 400+ Drug Targets, an expression focused informatics resource for 3,200 genes.

   LifeSpan Bioscience  
         
  15.04.2008 AnaSpec      
 

FRET brochure from AnaSpec

      
 

The new FRET brochure from AnaSpec contains:

  • Biological Application of FRET - Protease Detection
  • FRET Quenchers and Donors
  • ACE2 (Angiotensin Converting Enzyme) Assay Kits
  • HCV (Hepatitis C Virus) Assay Kits
  • HIV (Human Immunodeficiency Virus) Assay Kits
  • MMP (Matrix Metalloproteinasen) Assay Kits
  • Renin Assay Kits
  • TACE (TNF-α Converting Enzyme) or α-Secretase Assay Kit
  • β-Secretase Assay Kit
  • WNV (West Nile Virus) Assay Kit
  • Other FRET Substrates
  • FRET Building Blocks
  • Guidelines in Designing FRET Peptide Substrates
Download the new FRET Brochure as PDF (400 kb) or order a free paper Copy!

  AnaSpec FRET Brochure  
         
     
  top  
         

home | search | site map | order | Impressum |

©2010 MoBiTec GmbH • All rights reserved • www.mobitec.com