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TaKaRa

The Japanese company Takara Bio is committed to preventing disease and improving the quality of life of people around the world through the use of biotechnology.

For more information please visit the TaKaRa Logo Takara webpage.
 

Below you will find some selected Takara products.

   TaKaRa logo


TaKaRa's Taq Polymerases, see below
TaKaRa's PrimeScript™ reverse transcriptase
TaKaRa's Cell Biology Products
TaKaRa PCR Thermal Cycler Dice
TaKaRa's pCOLD expression vectors
Restriction Enzymes


Please download:

Premium Products Selection (pdf 3.1 MB)

This selection covers TaKaRa's range of enzymes and premixes for Real Time PCR, RT-PCR and PCR as well as pCold vectors, Chaperone plasmids and Refolding CA kit.



Choose your PCR enzyme
TaKaRa PrimeSTAR™
TaKaRa SpeedSTAR™
TaKaRa Ex TaqTM
TaKaRa LA TaqTM
TaKaRa Ex TaqTM Hot Start Version
TaKaRa Ex TaqTM R-PCR Version
Premix Ex TaqTM (Perfect Real Time PCR)
SYBR® Premix Ex Taq (Perfect Real Time PCR)
TaKaRa Z-Taq™

How to select the Best PCR Enzyme

sheme: how to select the best PCR enzyme

For more information how to select the best PCR enzyme
please download pdf (70 kb)

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TaKaRa PrimeSTAR™

PrimeSTAR™ HS DNA Polymerase, a novel new high fidelity PCR enzyme which provides maximum fidelity as well as extended product length (8.5 kb for human genomic DNA; 22 kb for l DNA). Targeted for demanding cloning (i.e. amplification of cDNA libraries) and sequencing applications, PrimeSTAR™ HS offers extremely high accuracy, and is the only enzyme whose fidelity is calculated by the method you use - sequence analysis. It also offers excellent amplification efficiency and shortened reaction times.

PrimeSTAR™ polymerase offers:

  • High Accuracy: A strong exonuclease activity results in an extremely low error rate, with only 12 errors per 250,000 bp as determined by DNA sequence analysis.

  • High Efficiency: Amplification efficiency higher than Taq olymerase.

  • Robust Amplification: Tolerance to varying reaction conditions means a single PCR cycling protocol can be used to amplify products of varying sizes.

  • GC-Rich Targets: Robust performance even with GC-rich templates.

More information on TaKaRa's PrimeSTAR™

ORDER INFORMATION:
order# description amount 
R010A-TK PrimeSTAR™ HS DNA polymerase 250 U
R010B-TK PrimeSTAR™ HS DNA polymerase 1000 U
R010Q-TK PrimeSTAR™ (Sample size) 50 U
shipped on dry ice, store at -20°C

 
PrimeSTAR™ Fidelity Comparison with Other DNA Polymerases and Taq.
PrimeSTAR™ Fidelity Comparison with Other DNA Polymerases and Taq.
PrimeSTAR: A Single PCR Cycling Protocol Produces Optimal Amplification of Fragments of Varying Sizes:
A Single PCR Cycling Protocol Produces Optimal Amplification of Fragments of Varying Sizes:
1. DCLRE1A 4 Kb; 2. beta-globin 8.5 Kb; 3. beta-globin 6 Kb; 4. DCLRE1A 2 Kb; 5. p53 0.5 Kb; 6. p53 4 Kb; 7. beta-globin 7.5 Kb; 8. DCLRE1A 8 Kb;
9. DCLRE1A 1 Kb; 10. p53 6 Kb; M1: lambda-Hind III-digest; M2 : pHY Marker
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TaKaRa SpeedSTAR™ for High-Speed PCR

SpeedSTAR™ HS DNA Polymerase is a convenient, efficient DNA polymerase specially designed for fast PCR. Extension times of as short as 10 sec/kb are possible, (compared to 60 sec/kb with general enzymes), dramatically reducing total reaction times. SpeedSTAR™ reactions can be performed using standard PCR instruments, and the robust two-buffer system facilitates efficient amplification of varyingsize fragments (up to 20 kb) with less optimization than other polymerases. Additionally, the hot start formulation provides increased specificity and reduced background.

  • High Speed Amplification: Amplify a 2 kb fragment in as little as 30 minutes.

  • Excellent Efficiency: Robust performance, comparable to high yield polymerases.

  • Long Fragments: Optimized two-buffer system allows amplification of fragments up to 20 kb with reduced optimization.

  • No Special Instrument Needed: Cut reaction times by two-thirds without purchasing a specialized instrument.

More information on TaKaRa's SpeedSTAR™

ORDER INFORMATION:
order# description amount 
RR070A-TK SpeedSTAR HS DNA Polymerase 250 U
RR070Q-TK SpeedSTAR HS DNA Polymerase 50 U
shipped on dry ice, store at -20°C

 

    
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TaKaRa Ex TaqTM

Features and Advantages
  • Greater Processivity
  • Higher Fidelity
  • Greater Amplification Yield
Description

TaKaRa Ex TaqTM is the key enzyme for LA PCR Technology. TaKaRa Ex TaqTM provides more efficient amplification and higher fidelity than conventional Taq DNA polymerase under conventional PCR conditions.

Concentration
5 units/µl

Form
20 mM Tris HCl (pH8.0), 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5% Tween 20, 0.5% Nonidet P-40, 50% Glycerol

More information on TaKaRa's Ex TaqTM

Please note: Ex TaqTM sample size is available!!

ORDER INFORMATION:
order# description amount 
R010A-TK PrimeSTAR™ HS DNA polymerase 250 U
R010B-TK PrimeSTAR™ HS DNA polymerase 1000 U
R010Q-TK PrimeSTAR™ (Sample size) 50 U
RR001A-TK TaKaRa Ex Taq™(Mg2+ buffer) 250 U
RR001B-TK TaKaRa Ex Taq™(Mg2+ buffer) 1000 U
RR001C-TK TaKaRa Ex Taq™(Mg2+ buffer) 3000 U
RR001T-TK TaKaRa Ex Taq™ (sample size) 50 U
RR01AM-TK TaKaRa Ex Taq™(Mg2+ free buffer) 250 U
RR01BM-TK TaKaRa Ex Taq™(Mg2+ free buffer) 1000 U
RR01CM-TK TaKaRa Ex Taq™(Mg2+ free buffer) 3000 U
shipped on dry ice, store at -20°C
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TaKaRa LA TaqTM

Features and Advantages
  • Long and Accurate PCR (Cat.# RR002A/M-TK)
  • Suitable for Genomic DNA Amplification (Cat.# RR002A/M-TK)
  • Applicable to GC Rich Template (Cat.# RR02AG-TK)
Description

TaKaRa LA TaqTM is a thermostable DNA polymerase having 3' - 5' exonuclease activity (proof reading activity) utilizing LA PCR technology. Under general PCR conditions, higher efficiency and higher fidelity are obtained compared than conventional Taq DNA polymerases. This enzyme is basically applicable to any template DNA, and especially effective in amplifying more than 15 kbp fragments. For amplification of templates having complex secondary structure or high GC content, TaKaRa LA TaqTM with GC Buffer works the best. The supplied 2 x GC Buffer I, II are designed to achieve the best amplification of GC rich-template or ones having repeated sequences.

More information on TaKaRa's LA TaqTM

Please note: LA TaqTM sample size is available!!

ORDER INFORMATION:
order# description amount 
RR002A-TK TaKaRa LA TaqTM 125 U
RR002M-TK TaKaRa LA TaqTM 250 U
RR002T-TK TaKaRa LA TaqTM (sample size) 50 U
RR02AG-TK TaKaRa LA TaqTM with GC buffer 125 U
shipped on dry ice, store at -20°C
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TaKaRa Ex TaqTM Hot Start Version

Features and Advantages
  • Reduced Background
  • Increased Specificity
  • High Performance
Description

TaKaRa Ex Taq HS is designed to be suitable for Hot Start PCR. It is derived from TaKaRa Ex Taq and neutralizing monoclonal antibody to Taq DNA polymerase. Non-specific amplification due to mispriming and/or formation of primer dimer before thermal cycling can be prevented, since the antybody inhibits the polymerase activity by binding to the Taq DNA polymerase until the temperature elevates. This enzyme can be also used in general PCR conditions, since monoclonal antibody is denatured in the initial DNA-denaturation step.


More information on TaKaRa's Ex TaqTM Hot Start Version

Please note: Ex TaqTM Hot Start Version sample size is available!!

ORDER INFORMATION:
order# description amount 
RR006A-TK TaKaRa Ex TaqTM Hot Start Version 250 U
RR006B-TK TaKaRa Ex TaqTM Hot Start Version 1,000 U
RR006Q-TK TaKaRa Ex TaqTM Hot Start Version (sample size) 50 U
shipped on dry ice, store at -20°C
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TaKaRa Ex Taq R-PCR Version

TaKaRa Ex Taq R-PCR allows more accurate and higher sensitivity real time PCR. An innovative buffer system improves denaturation, thus minimizing non-specific amplification derived from primer dimer formation.
Improved conditions using Smart Cycler (Cepheid) and LightCycler (Roche) Systems

More information on TaKaRa's Ex TaqTM R-PCR

ORDER INFORMATION:
order# description amount 
RR031A-TK TaKaRa Ex TaqTM R-PCR Version 250 U
RR031B-TK TaKaRa Ex TaqTM R-PCR Version 1,000 U
shipped on dry ice, store at -20°C
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Premix Ex TaqTM (Perfect Real Time PCR)

Features and Advantages
  • Versatility: Compatible with Smart Cycler®, LightCycler®, ABI PRISM® (7000, 7700, 7900), RotorGene™ and other real time PCR instruments.

  • High sensitivity: Detects as few as 100 copies.

  • Wide dynamic range: Possesses a dynamic range of 7-8 orders of magnitude (λ DNA template).

  • Accurate quantification: Produces excellent standard curves using numerous real time instruments.

  • Convenient: A separate tube of ROX reference dye is supplied.


SYBR® Premix Ex Taq used with SMART Cycler®
Fig. above: SYBR® Premix Ex Taq used with SMART Cycler® (Cepheid)


Description

Premix TaqTM (Ex TaqTM R-PCR Ver.) is a premix reagent designed to be available for Real Time PCR. The polymerase used in this product is TaKaRa Ex TaqTM for Hot Start PCR which is mixed with the neutralizing monoclonal antibody to Taq DNA polymerase. It allows the best performance in real time PCR by combination of this polymerase and the optimized buffer. This premix reagent is also designed to use simply and easily.

Real time monitoring of PCR is performed by addition of SYBR® Green I or various probes (ex. TaqMan® Probe, Applied Biosystems) as a detection reagent, using an exclusive instrument (ex. Smart Cycler® System, Cepheid).


More information on Premix Ex TaqTM R-PCR

Free Samples available! please request...

ORDER INFORMATION:
order# description amount 
RR039A-TK Premix Ex Taq (Ex Taq R-PCR 2.1 Version) 200 tests
RR039B-TK Premix Ex Taq (Ex Taq R-PCR 2.1 Version) 400 tests
shipped on dry ice, store at -20°C
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SYBR® Premix Ex Taq (Perfect Real Time)

SYBR® Premix Ex Taq™ DNA Polymerase with ROX is a convenient (2X) premix consisting of Takara's high fidelity-high performance Ex Taq122; Hot Start DNA Polymerase, SYBR® Green I, and a newly formulated real time buffer which provides superior specificity and increased amplification efficiency for real time PCR. Antibody-mediated hot start technology prevents nonspecific amplification due to mispriming and/or formation of primer dimers during the reaction assembly. The Taq antibody-polymerase complex is denatured in the first cycling step, releasing the polymerase and allowing DNA synthesis to proceed.
Additionally, ROX reference dye is also supplied as a separate tube. ROX dye is a convenient internal reference standard for use in normalizing signals due to non-PCR related fluorescence fluctuations that occur either between wells or over time.

SYBR® Premix Ex Taq™ DNA Polymerase with ROX provides superior specificity, performance, and amplification yield for real time PCR on all major real time instruments.


More information on SYBR® Premix Ex Taq (pdf 541 kb)

Please note: SYBR® Premix Ex TaqTM sample size is available!!

For a detailed data sheet as a pdf-file, please click on the product No.
ORDER INFORMATION:
order# description amount 
RR041A-TK SYBR® Premix Ex TaqTM 200 tests
RR041B-TK SYBR® Premix Ex TaqTM 200 tests x 2
RR041Q-TK SYBR® Premix Ex TaqTM(sample size) 40 tests
shipped on dry ice, store at -20°C
go order  


TaKaRa Z-Taq™

For high-speed PCR
Features and Advantages
  • Five-time Faster Speed than Taq Polymerase
  • Up to 17.5 kbp Amplification of Human Genomic DNA
  • Suitable for Multiple-sample Processing
  • Time- and Cost- Effective
Description

TaKaRa Z-TaqTM is a DNA polymerase especially developed to achieve rapid PCR. It offers PCR productivity unmatchable by any other enzyme, at 5-time faster speed. It achieves amplification of 1 kbp within 20 minutes. In addition to this high speed, specificity and sensitivity is as high as TaKaRa Ex TaqTM. It enables amplification of 17.5 kbp of human genome DNA. Thanks to its high productivity, TaKaRa Z-TaqTM is the best suitable for highthroughput multi-sample processing on a single cycler. By increasing cycling numbers (up to 50 cycles), the sensitivity can be further improved. Therefore, TaKaRa Z-TaqTM is highly efficient and cost-effective, which can accelerate genetic applications in every related field.

More information on TaKaRa Z-TaqTM

Please note: TaKaRa Z-TaqTM sample size is available!!

ORDER INFORMATION:
order# description amount 
RR070A-TK SpeedSTAR HS DNA Polymerase 250 U
RR070Q-TK SpeedSTAR HS DNA Polymerase 50 U
R006A-TK TaKaRa Z-TaqTM 200 U
R006B-TK TaKaRa Z-TaqTM 800 U
R006Q-TK TaKaRa Z-TaqTM (sample size) 50 U
shipped on dry ice, store at -20°C
go order  




Would you like to learn more about Takara products?

Please request a free catalog.

   TaKaRa catalog
     

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